Part:BBa_K2372005:Design

GyrB^R from Staphylococcus aureus with terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 511
  Illegal AgeI site found at 1459
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1262
  

Design Notes

Using this part to gain resistance in E.coli only makes sense in combination with GyrA from S.aureus, because E.coli GyrA will not interact with this GyrBR variant.

Source

Staphylococcus aureus